Posted 1 month ago

Research Assistant – Physiology

Company: Midwestern University
Category: Professional Services

 Downers Grove

Type: Full Time

The Department of Physiology at Midwestern University is looking for a motivated, enthusiastic, and scientifically curious Research Assistant to provide research support for faculty members in the Department of Physiology. The Research Assistant is expected to assist in the: design and execution of animal, cellular, and human based research projects; data collection and analysis; and preparation of figures for manuscripts for peer-reviewed publications. The candidate should have experience working with laboratory mice. This position will report to the Chair of the Department.
Essential Duties and Responsibilities:

  • Maintain transgenic mouse colonies under faculty supervision: Mice breeding, handling, ear tagging, drug delivery via intraperitoneal, intramuscular, or subcutaneous injections, genotyping (obtain tail snips and perform PCR analyses), and accurate maintenance of colony records.
  • Cell culture maintenance under faculty supervision: Generation, propagation and maintenance of sterile cell cultures and intestinal organoids, performing basic in vitro experiments, including but not limited to harvesting, splitting, and freezing cells, drug delivery to cultures, protein, and RNA extractions.
  • Competently perform standard molecular biology techniques (western blot, gel electrophoresis, RT-PCR, qPCR, chromatin immunoprecipitations, immunohistochemistry, molecular cloning, etc.) under faculty supervision.
  • Use specialized computer software to collect and analyze physiological (cardio-pulmonary) and cognitive data from humans under faculty supervision.
  • Graph, analyze, and evaluate generated data, and contribute to abstract submissions and poster preparation.
  • Maintain an accurate lab notebook and assist with trouble-shooting technical issues.
  • Assist in literature searches for protocol development and optimization.
  • Maintain cleanliness and organization of lab, lab equipment, and supplies.
  • Adhere to laboratory safety and regulatory procedures and maintain lab chemical inventory.
  • Obtain quotes and perform ordering and receiving duties for lab supplies.